Real Madrid forward Gareth Bale will miss Wales’ friendly against the Netherlands in Cardiff on November 13.
Bale has not played since October 17 after suffering a recurrence of a calf injury following the October internationals.
The 26-year-old last featured in the first half of Real’s 3-0 victory over Levante four days after Wales’ Euro 2016 qualification party in Cardiff.
Arsenal’s Aaron Ramsey picked up a hamstring injury and also misses Wales’ first Euro 2016 warm-up game at the Cardiff City Stadium.
Reading forward Hal Robson-Kanu and Fulham defender Jazz Richards are other absentees through injury.
Newcastle defender Paul Dummett and Walsall striker Tom Bradshaw have been called into the squad.
Goalkeepers: Wayne Hennessey (Crystal Palace/ENG), Danny Ward (Liverpool/ENG), Owain Fon Williams (Inverness/SCO)
Defenders: Ashley Williams (Swansea City), James Chester (West Brom/ENG), Chris Gunter (Reading/ENG), Neil Taylor (Swansea City), Ben Davies (Tottenham Hotspur/ENG), James Collins (West Ham United/ENG), Adam Henley (Blackburn Rovers/ENG), Paul Dummett (Newcastle/ENG) (Fulham/ENG)
Midfielders: David Vaughan (Nottingham Forest/ENG), Emyr Hughes (Wigan/ENG), Joe Allen (Liverpool/ENG), Joe Ledley (Crystal Palace/ENG), Andy King (Leicester City/ENG), David Edwards (Wolverhampton Wanderers/ENG), Jonathan Williams (Crystal Palace)
Forwards: David Cotterill (Birmingham City/ENG), Tom Lawrence (Leicester City/ENG), Simon Church (MK Dons/ENG), Sam Vokes (Burnley/ENG), Tom Bradshaw (Walsall/ENG)
. By AFP